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What is the difference between transfection, transduction, and transformation?

Hey, guess what!? Just to make science more confusing there are multiple definitions for each. Definition 1 is the one that’s relevant for this class.

 

Transfection = (1) introduction of DNA or RNA into eukaryotic cells and (2) introduction of a bacteriophage into bacterial cells.

 

Transformation = (1) uptake of DNA from the environment by a bacterial cell and (2) in eukaryotes, the conversion of normal cells into tumor cells

 

Transduction = (1) infection of mammalian cells with a viral vector

 

Note: “Transduction is not a biology-specific term. The common definition of transduction is ‘leading through or across.’ The term has been used to describe DNA transfer between bacterial cells by bacteriophage. However, in current research, transduction also refers to the infection of mammalian cells with a viral vector. ‘Transduction’ was first used to describe bacterial gene transfer back in the 1950s, decades before the creation of viral vectors. It’s possible that the word has been co-opted to prevent confusion, as ‘transfection’ doesn’t distinguish between the introduction of nucleic acid or viral particles into a cell. ‘Transduction’ is mostly used to describe the introduction of recombinant viral vector particles into target cells, while ‘infection’ refers to natural infections of humans or animals with wild-type viruses. There are no universally accepted differences between these two terms (a quick Google search uncovers all kind of online debate).  They are mostly used as a convenient way to differentiate between two biological mechanisms.” https://bitesizebio.com/32346/introducing-foreign-dna-terminology/

 

Is DNA transfer via liposomes possible in prokaryotes?

I asked a microbiologist this question and was told that 1) competent cells are so easy to make that why bother using expensive liposomal transfection reagents and that 2) the cell wall and different polysaccharides may impeded the liposomal transfection anyway.

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